The goal of the project is to fabricate the integrated ion sensitive field effect transistors and to develop sensing platform on the scleral lenses. This project is mainly divided into three parts. The first part entitled “The establishment and design of the scleral lens sensing platform”. In general, the traditional soft contact lenses were used as the sensing platform. In this project, the rigid scleral lenses are used to replace traditional soft contact lenses and to improve the reliability of measurement environment. To crate the stable measurement environment of the precorneal fluid reservoir, the structure of rigid scleral lenses was designed by using the back optic zone radius (BOZR), back optic zone diameter (BOZR), and back scleral radius (BSR). Furthermore, the tear fluid diffusion flow are observed by using the fluorescein test method. The second part entitled “Fabrication and investigation of the biosensors by using ion sensitive field effect transistors”. In the fabrication of the field-effect-transistors, a n-type AlGaZnO and n+-type ZnO thin film were deposited by using the RF magnetron sputtering. To improve and optimize the performance of the field-effect-transistors, the influence of power ratio to the characteristic was investigated. Finally, the AlGaZnO sensing membrane was deposited and integrated on the field-effect-transistors to form the ion-sensitive field-effect transistor (ISFET). The sensing performance of the various pH value in the eye was measured and analyzed by using the simulated solution of the tear. The third part entitled “The biosensor devices of the ZnO nanorods/AlGaZnO based ISFET” utilizes the various density and the space of the nanorods was used to increase the surface-to-volume ratio and to improve the performance of the biosensor. Finally, the biosensor of the ZnO nanorods/AlGaZnO based ISFET will be integrated and constructed with the sensing platform of the scleral lenses.